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Product ID: 800-8357

BD Biosciences Accuri C6 Flow Cytometer

BD Biosciences Accuri C6 Flow Cytometer

Includes: 

  • Recent PM
  • Computer loaded with Accuri Software
  • BD CSampler Accessory
  • (2) Laser Configuration

Specifications:

Optics

  • Laser Excitation
  • 488 nm (rated at 20,000-h life)
  • 640 nm (rated at 20,000-h life)
  • Laser Profile: 10 x 75 μm
  • Light Scatter Detection
  • Forward (0°, ±13°)
  • Side (90°, ±13°)
  • Emission Detection
  • 4 colors, user-changeable optical filters
  • Standard set installed:
  • FL1 533/30 nm (eg, FITC/GFP)
  • FL2 585/40 nm (eg, PE/PI)
  • FL3 > 670 nm (eg, PerCP,PerCP-Cy5.5, PE-Cy™7)
  • FL4 675/25 nm (eg, APC)
  • Optical Alignment: Fixed alignment

Fluidics

  • Flow Cell: 200 μm ID quartz capillary
  • Minimum Detectable Particle Size: 0.5 μm
  • Minimum Sample Volume: 50 μL
  • Pre-Set Flow Rates and Core Sizes
  • Slow : 14 μL/min, 10-μm core
  • Medium: 35 μL/min, 16-μm core
  • Fast: 66 μL/min, 22-μm core
  • Custom Sample Flow Rates: 10–100 μL/min
  • Custom Core Diameter: 5–40 μm
  • Recommended Sheath Fluid: 0.2-μm filtered DI water
  • Maximum Events Per Sample: 1 million events per well

Performance

  • Fluorescence Sensitivity, MESF*: FITC < 150; PE < 100
  • Scatter Resolution: Resolves human peripheral blood lymphocytes, monocytes, and granulocytes
  • Fluorescence Linearity: 2 ±0.05% for chicken erythrocyte nuclei (CEN)
  • Fluorescence Precision: <3% CV for CEN
  • Data Acquisition Rate: 10,000 events/second, maximum

BD CSampler Accessory

  • Power Requirements: No additional power necessary
  • Software Requirements: BD CSampler™ software
  • Compatible Plate Types
  • Standard 96-well (flat, round, and v-bottom) plates
  • Deep-well 96-well plates
  • 48-well plates
  • 12 x 75-mm tubes can also be accommodated using the supplied 24-tube rack.
  • Plates manufactured to the guidelines published by the American National Standards Institute, submitted by the Society for Biomolecular Screening, should be compatible with the BD CSampler.
  • Space Requirements
  • Minimum bench depth: 28 in. (71 cm)
  • Minimum width (C6 with CSampler): 19.5 in. (49.5 cm)
  • Processing Time
  • <90 minutes for 96-well plate, utilizing
  • 30-second acquisition time per well
  • Weight: 7 lb (3.2 kg)
  • Minimum Sample Volume:50 μL for tubes or plates
  • Wash Cycle: Up to 3 wash cycles per well
  • Agitate Cycle: Up to 3 agitate cycles per well

Additional Details

  • Manufacturer: BD Biosciences
  • Model Name: Accuri C6
  • Model Number:
  • Product ID: 800-8357
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