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Product ID: 11370

BD Biosciences LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen Flow Cytometer

BD Biosciences LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen Flow Cytometer

Recent PM from BD (11/16/22); Certified and Ready for a Service Contract! 


  • HTS (High Throughput Options)
  • Windows 7 Pro computer with FACSDiva Software Version 8.0.1
  • Installed Lasers:
  • Violet Laser 405 nm: 50 mW with 2 PMT detectors (Trigon)
  • 525/50 (505LP)
  • Z473/10
  • Blue 488 nm: 50 mW with 3 PMT detectors plus side scatter (Octagon)
  • 710/50 (685LP)
  • 582/15 (570LP)
  • 488/10
  • 675/20 (670LP)
  • 515/20 (505LP)
  • Yellow Green 561 nm: 50 mW with 3 PMT detectors (Octagon)
  • 610/20 (600LP)
  • 670/630 (635LP)
  • 582/15

Technical Specifications:

  • Excitation Optics
  • Excitation Optical Platform:The special order BD LSRFortessa optical layout allows for up to five lasers.
  • Flow Cell Design:Rectangular Quartz Cuvette: Internal cross-section, 430 x 180 μm
  • Fixed optical assembly of the lasers, with up to 5 spatially-separated laser beams.

Emission Optics

  • Optical Coupling:The quartz cuvette flow cell is gel coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
  • Forward Scatter Detection:Photodiode detector with a 488/10 bandpass filter
  • Side Scatter Detector:Photomultiplier tube (PMT) with a 488/10 bandpass filter
  • Emission Optical Design:The special order BD LSRFortessa cell analyzer uses BD’s patented octagon and trigon detection system. Using reflective optics and fiber-coupled PMTs ensures more efficient light collection than that obtained from transmission optics.


  • Fluorescence Sensitivity:FITC-A: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE-A: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
  • Measurements performed using SPHERO Rainbow Calibration Particles RCP-30-5A
  • Fluorescence Resolution:Coefficient of variation PI: area of <3%, full G0/G1 peak for chicken erythrocyte nuclei (CEN) stained with propidium iodide (PI)
  • Fluorescence Linearity:Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
  • Side Scatter Sensitivity:Achieves separation of 0.5-µm beads from noise in side scatter.
  • Forward and Side Scatter Resolution:Scatter performance is optimized for resolution of lymphocytes, monocytes, and granulocytes.
  • Forward Scatter PMT Option:A forward scatter PMT upgrade is available for small particle detection through the special order program.
  • Data Acquisition Rate:Theoretical maximum electronic data collection of 70,000 events/second.
  • Typical operation at 20,000 events/second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min


  • Sample Flow Rates:Front key panel provides three modes: RUN, STNDBY, and PRIME
  • Continuously adjustable flow rate, plus three preset flow rates:LO = 12 μL/min
  • MED = 35 μL/min
  • HI = 60 μL/min
  • Standard Fluidic Reservoirs:One 8-L sheath and 10-L waste container provided.


Additional Details

  • Manufacturer: BD Biosciences
  • Model Name: LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen
  • Model Number:
  • Product ID: 11370
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