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Product ID: 12109

BD Biosciences LSRFortessa X20 SORP 4 Lasers: Blue/Red/Violet/YellowGreen Flow Cytometer

BD Biosciences LSRFortessa X20 SORP 4 Lasers: Blue/Red/Violet/YellowGreen Flow Cytometer

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Includes:

  • HTS (High throughput option)
  • Windows computer with FACSDiva Software
  • Installed Lasers:
  • Blue: 488 nm
  • Red: 640 nm
  • Violet: 405 nm
  • Yellow/Green: 561nm

The BD LSRFortessa X-20 cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization.

The instrument delivers the optimal sensitivity and resolution required for multicolor applications. The BD LSRFortessa X-20 cell analyzer can be used to detect up to 18 colors simultaneously and supports up to 5 lasers. In addition to the reduced size, design innovations make filters and detectors more accessible for easier setup of new experiments.

 

Specifications:

Optics

Excitation Optics: 

  • Excitation Optical Platform: The BD LSRFortessa X-20 optical layout allows for up to five lasers.
  • Laser Power Options:
  • 355 nm: 15 mW
  • 405 nm: 50 mW
  • 488 nm: 50 mW
  • 561 nm: 50 mW
  • 640 nm: 40 mW
  • Optical Efficiency: Power loss at flow cell: <20% of specified laser power
  • Flow Cell Design
  • Rectangular quartz cuvette:Internal cross-section, 430 x 180 μm
  • External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light.
  • Fixed optical assembly with spatially separated laser beams.

Emission Optics

  • Optical Coupling: The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal
  • collection efficiency.
  • Forward Scatter Detection: Photodiode detector with a 488/10 bandpass (BP) filter
  • Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
  • Emission Optical Design:Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa X-20 uses configurable polygonshaped optical pathways that use signal reflection to maximize signal detection. Please see the filter guide at bdbiosciences.com for information on dye and filter options.

Performance

  • Fluorescence Sensitivity
  • FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
  • PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5)
  • APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
  • FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A)
  • PE-Cy5 and APC measurements performed using SPHERO™ Ultra Rainbow Calibration Particles (URCP38-2K)
  • Fluorescence Resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Fluorescence Linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
  • Forward and Side Scatter Sensitivity: Enables separation of fixed platelets from noise.
  • Forward and Side Scatter Resolution: Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
  • Side Scatter Resolution: Enables separation of 0.5-μm beads from noise.
  • Forward Scatter PMT Option: A forward scatter PMT upgrade is available for small
  • particle detection. Requires the 100-mW, 488-nm blue laser.
  • Data Acquisition Rate: 40,000 events/sec with beads

Fluidics

  • Sample Flow Rates
  • Front button panel provides three modes: RUN, STANDBY, and PRIME
  • Continuously adjustable flow rate, plus three preset flow rates:
  • LO: 12 μL/min
  • MED: 35 μL/min
  • HI: 60 μL/min
  • Standard Fluidic Reservoirs: One 8-L sheath container and one 10-L waste container provided.
  • Recommended Fluidics Option: BD FACSFlow supply system: automated fluidics system, which includes a rolling cart and two 20-L Cubitainer packages

Options

Data Management

  • Software: BD FACSDiva v8.0 or later
  • High Throughput Option: The BD High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate. The HTS can be front or side mounted on the BD LSRFortessa X-20.
  • HTS Throughput Acquisition:Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second acquisition, less than 44 minutes in standard mode using a 10-second acquisition
  • Carryover:
  • <0.5% High-throughput mode
  • <0.75% Standard mode

Installation Requirements

  • Dimensions (H x W x D):76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.)
  • Weight:~159 kg (~350 lb) depending on configuration
  • Temperature Operating Range: 66ºF–79ºF (19ºC–26ºC)
  • Humidity:10% to 90% relative, non-condensing
  • Heat Dissipation: ~2,700 BTU per hour depending on configuration
  • Power
  • Operation at 100/120/230 VAC and 50 or 60 Hz
  • Maximum power:1,500 watts
  • Noise:<70 dB
  • Air Supply:None required
  • Electrical Requirements:BD requires one dedicated circuit for the cytometer and the computer system (including printer) with a dedicated AC source not shared with any other equipment. The instrument will be powered from the line conditioner supplied by BD Biosciences.

Additional Details

  • Manufacturer: BD Biosciences
  • Model Name: LSRFortessa X20 SORP 4 Lasers: Blue/Red/Violet/YellowGreen
  • Model Number:
  • Product ID: 12109
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