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Product ID: CA-12383

Bio-Rad ZE5 Cell Analyzer, 5 laser 27 Color (Red/Blue/Violet/Yellow-Green/UV) Flow Cytometer

Bio-Rad ZE5 Cell Analyzer, 5 laser 27 Color (Red/Blue/Violet/Yellow-Green/UV) Flow Cytometer


  • Computer loaded with software
  • Lasers:
  • 355 nm (UV)
  • 405 nm (violet)
  • 488 nm (blue)
  • 561 nm (yellow-green)
  • 640 nm (red)


  • Fluorescence sensitivity: Average MESF: FITC: <70 PE: <50 and APC: <40
  • Scatter sensitivity: <0.2 μm FSC resolution with small-particle detection module
  • Sample loading:
  • 96-well and 384-well microplates, standard or deep-well
  • 40-tube rack of 5 ml tubes (12 x 75 mm)
  • 24-tube rack of microcentrifuge tubes
  • STAT tube position for single tube loading
  • Sample throughput: <15 min for 96-well plate and < 60 min for 384-well plate in high-throughput mode
  • Detection: Up to 30 detectors, including FSC and SSC; optional second FSC detector
  • Cuvette: Fused silica with 145 x 265 μm channel
  • Speed: Up to 100,000 events per second with all parameters enabled
  • Data processing
  • Simultaneous measured peak, area, and width for every channel
  • 24-bit data for area and height
  • 17-bit data for width with high-resolution linear interpolation at the half height
  • Fluidics:
  • Bulk fluids: 4 x 4 L bulk fluid tanks on board for sheath and waste
  • Onboard additive concentrate and cleaner
  • Operationalflow rates: 0.025–3.5 μl/sec
  • Dimensions(W x D x H) : 74 x 69 x 66 cm (29 x 27 x 26 in)
  • Weight: 110 kg (240 lb) maximum
  • Data format:Flow Cytometry Standard (FCS) 3.1


The ZE5 Cell Analyzer is an innovative, versatile flow cytometer designed to deliver powerful advancements in the quality and quantity of data that you can generate. It is versatile enough to handle complex assays and able to deliver data at a rate unmatched by other instruments.


The ZE5 Cell Analyzer has a host of attributes that make it perfect for a wide range of applications and workflows:

  • Allows complex immunophenotyping with up to 5 lasers and 30 detection parameters
  • Adapts to your workflow, with a universal sample loader that can accommodate a wide range of sample formats and has integrated vortexing and temperature control
  • Powers through high throughput and screening assays with fast plate handling, allowing 96-well plates to be completed in less than 15 minutes and 384-well plates in less than 60 minutes
  • Detects rare events faster with an acquisition rate of up to 100,000 events/second without losing data due to electronic aborts
  • Resolves small particles, including exosomes, with a dedicated small particle detector
  • Performs advanced analysis with FCS Express analysis software

Easy to Use

Designed for busy multi-user laboratories and with an emphasis on ease of use, it incorporates innovative features that make it ideal for users with any level of experience:

  • Learn, use, and train with ease with intuitive software and a unique experiment builder wizard
  • Fluidics that never run dry with dry sample detection and live sheath and waste tank monitoring
  • Self-cleaning with on-board cleaner
  • QC at the touch of a button with on-board QC beads

Automation and 21 CFR Part 11 Ready

If you are considering flow cytometry for high throughput screening or pre-clinical applications that require 21 CFR Part 11 compliance, the ZE5 Cell Analyzer is an ideal choice.

  • Designed to allow easy integration into robotic workcells
  • Hands-off operation with optional large capacity fluidics
  • Widely compatible with new or existing robotic workcells with a hardware and software agnostic application programming interface (API)
  • Data acquisition with 21 CFR Part 11 support using Xybion Compliance Builder software
  • Advanced data analysis with 21 CFR Part 11 support with FCS express analysis software

Additional Details

  • Manufacturer: Bio-Rad
  • Model Name: ZE5 Cell Analyzer, 5 laser 27 Color (Red/Blue/Violet/Yellow-Green/UV)
  • Model Number: 355/405/488/561/640
  • Product ID: CA-12383
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