Zeiss LSM 780 Laser Scanning Confocal System w/ Zeiss Observer Z1 Inverted Phase Contrast Fluorescence Microscope

Optional Installation and Service Contract

LSM780 Specifications:

Scanning Module

• Models: Scanning module with 34 spectral detection channels; high QE (45% for GaAsP typically), 3 × lower dark noise; up to 10 individual, adjustable digital gains; prepared for lasers from UV to IR
• Scanners: Two independent, galvanometric scan mirrors with ultra-short line and frame flyback
• Scan resolution: 4 × 1 to 6144 × 6144 pixels; also for multiple channels; continuously variable 
• Scanning speed: 15 × 2 speed stages; up to 8 frames/sec with 512 × 512 pixels (max. 250 frames/sec 512 × 16); up to 4000 lines per second 
• Scan zoom: 0.6 × to 40 ×; digital variable in steps of 0.1 (on Axio Examiner 0.67 × to 40 ×)
• Scan rotation: Free rotation (360 degrees), in steps of 1 degree variable; free xy offset 
• Scan field: 20 mm field diagonal (max.) in the intermediate plan, with full pupil illumination 
• Pinholes: Master pinhole pre-adjusted in size and position, individually variable for multi-tracking and short wavelengths (e.g. 405 nm) 
• Beam path: Exchangeable TwinGate main beamsplitter with up to 100 combinations of excitation wavelengths and outstanding laser light suppression; optional laser notch filters for fluorescence imaging on mirror-like substrates (on request); outcoupling for external detection modules (e.g., FCS, B&H FLIM); low-loss spectral separation with recycling loop for internal detection
• Spectral detection: Standard: 34 simultaneous confocal fluorescence counting channels with highly sensitive low dark noise PMTs (2x) and GaAsP (32x); spectral detection range freely selectable (resolution down to 3 nm); in addition, two incident light channels with APDs for imaging and single photon measurements; transmitted light channel with PMT; cascadable non-descanned detectors (NDD) with PMT or GaAsP NDD unit for Axio Examiner and Axio Observer
• Data depth: 8-bit, 12-bit, or 16-bit selectable; up to 37 channels simultaneously detectable

Laser Inserts

• Laser inserts (VIS, V): (VIS, V, In Tune) pigtail-coupled lasers with polarization preserving single-mode fibers; stabilized VIS-AOTF for simultaneous intensity control; switching time < 5 μs, or direct modulation; up to 6 V/VIS-laser directly mountable in the scanning module; diode laser (405 nm, CW/pulsed) 30 mW; diode laser (440 nm, CW+pulsed) 25 mW; Ar-laser (458, 488, 514 nm) 25 mW or 35 mW; HeNe-laser (543 nm) 1 mW; DPSS-laser (561 nm) 20 mW; HeNe-laser (594 nm) 2 mW; HeNe-laser (633 nm) 5 mW (pre-fiber manufacturer specification) 
• External lasers (NLO, VIS, UV/V): Prepared laser ports for system extensions; direct coupling of pulsed NIR lasers of various manufacturers (including models with prechirp compensation); fast intensity control via AOM; NIR-optimized objectives and collimation; fiber coupling (single-mode polarization preserving) of external In Tune Laser, (488-640nm, <3nm width, pulsed) 1,5mW and prepared for UV laser (355nm, 60mW), manipulation lasers of high power in the VIS range 488–561 nm (e.g., LSM 7 DUO-systems)

Electronics Module 

• Real-time electronics: Control of the microscope, the lasers, the scan module and other accessory components; control of the data acquisition and synchronization by real-time electronics; oversampling readout logic for best sensitivity and 2 × better SNR; data communication between real-time electronics and user PC via Gigabit-Ethernet interface with the possibility of online data analysis during image acquisition 
• User PC: Workstation PC with abundant main and hard disk memory space; ergonomic, high-resolving 16:10 TFT flat panel display; various accessories; operating system Windows VISTA 32 or 64-bit; multi-user capable

Observer Z1 Includes:

• Trinocular AxioObserver Z1 base w/ Camera Port
• Optional Definite Focus FC12
• Eyepieces: (2)x Pl 10/23
• Illumination:
• Brightfield
• Phase Contrast
• NA:0.55 WD:26mm Phase Contrast Wheel
• Fluorescence
• Illuminator (Choose One):
• 100w Mercury w/ Power Supply
• Upgrade to: Used Metal Halide
• Upgrade to: New X-Cite 110LED System with Adapter X-Cite 110LED, speedDIAL remote, 3mm x 1500mm Liquid Light Guide
• Upgrade to: New X-Cite mini + System Includes: LED head, miniCUBE controller, speedDIAL remote Includes: – LED head, miniCUBE controller, speedDIAL remote, – Microscope Coupling Flange
• Upgrade to: New X-Cite XYLIS, XT720S w/1.5m LLG, Includes: – X-Cite XYLIS (XT720S), speedDIAL remote, 3mm x 1500mm Liquid Light Guide
• Filters (Additional Configurations Available)
• DAPI
• FITC
• CY3
• Objectives (Additional Configurations Available):
• Plan Neofluar Ph1 5x/0.15
• EC Plan-NEOFLUAR 10x/0.3
• LD Plan-NEOFLUAR/20x/0.4 Ph2 Korr
• LD Plan-NEOFLUAR 40x/0.6 Korr
• Type of Stage: Motorized Stage
• Motorized Components:
• Condenser
• Objective Nosepiece
• Filter Holder
• Z Drive

Configuration shown is for demonstration purposes. Contact us to discuss your application and needs for a customized quote.